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Absence of mutations in the NBS1 gene in B-cell malignant lymphoma patients
1Department of Radiation Biology, Hiroshima University, Japan. hns@mcai.med.hiroshima-u.ac.jp
Background:
Nijmegen breakage syndrome (NBS), also known as ataxia-telangiectasia (AT) variant, is an autosomal recessive disorder characterized by microcephaly, growth retardation, severe combined immunodeficiency and a high incidence of lymphoid carcinoma, the majority of which are B-cell lymphomas. To determine whether the NBS1 gene is a tumor suppressor gene in B-cell lymphoma, we screened B-cell malignant lymphoma (ML) for any evidence of NBS1 mutation.
Materials And Methods:
Sequence analysis of the NBS1 gene was performed from PCR products amplified from the DNA of 12 extracranial ML or RT-PCR products amplified from cDNA of 8 primary central nervous system lymphoma.
Results:
Direct sequence analysis revealed that no NBS1 mutations were present in any of these patients.
Conclusion:
The present results suggested that the contribution of NBS1 mutations to B-cell ML was minimal, despite the fact that the NBS1 gene was causative factor in these cases.