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High-risk corneal allografts are capable of stimulating complement dependent cytolytic antibodies
S L Hargrave1, S Taherzadeh, S Hegde
1Department of Ophthalmology, University of Texas, Southwestern Medical Center, Dallas 75235-9057, USA.
Purpose:
To prospectively characterize serum antibody responses in high-risk corneal allografts.
Methods:
Orthotopic and heterotopic C3H (H-2k) corneas were transplanted to CB6F1 (H-2b/d) recipient mice. Individual serum samples were collected at days 7, 14, 21, and 28 from orthotopically and heterotopically grafted mice. Samples were tested for anti-C3H antibodies using an enzyme-linked immunosorbent assay (ELISA). Serum samples were also tested for the ability to lyse target cells in the presence of complement.
Results:
In high-risk donor-host combinations, 100% corneal allograft rejection occurs in the absence of IgG1 production. Both orthotopic and heterotopic corneal allografts induced cytolytic complement fixing antibody isotypes.
Conclusions:
Allospecific corneal antibody may be involved in corneal graft rejection in high-risk donor-host combinations.