Gender differences in sweat lactate
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Updated: Oct 7, 2026

Quantitative Autonomic Testing
Published on: July 19, 2011
J M Green1, P A Bishop, I H Muir
1Department of Human Performance Studies, University of Alabama, Tuscaloosa 35487, USA. matt.green@wku.edu
Abstract:
Sweat rate may affect sweat lactate concentration. The current study examined potential gender differences in sweat lactate concentrations because of varying sweat rates. Males (n = 6) and females (n = 6) of similar age, percentage body fat, and maximal oxygen consumption (VO2max) completed constant load (CON) cycling (30 min--approximately 40% VO2max) and interval cycling (INT) (15 1-min intervals each separated by 1 min of rest) trials at 32 (1) degrees C wet bulb globe temperature (WBGT). Trials were preceded by 15 min of warm-up (0.5 kp, 60 rpms) and followed by 15 min of rest. Blood and sweat samples were collected at 15, 25, 35, 45, and 60 min during each trial. Total body water loss was used to calculate sweat rate. Blood lactate concentrations (CON approximately equal to 2 mmol.l-1, INT approximately equal to 6 mmol.l-1) and sweat lactate concentrations (CON and INT approximately equal to 12 mmol.l-1) were not significantly different (P > 0.05) at any time between genders for CON or INT. Overall sweat rates (ml.h-1) were not significantly different (P > 0.05) between trials but were significantly greater (P < or = 0.05) for males than for females for CON [779.7 (292.6) versus 450.3 (84.6) ml.h-1] and INT [798.0 (268.3) versus 503.0 (41.4) ml.h-1]. However, correcting for surface area diminished the difference [CON: 390.7 (134.4) versus 277.7 (44.4) ml.h-1, INT: 401.5 (124.1) versus 310.6 (23.4) ml.h-1 (P < or = 0.07)]. Estimated total lactate secretion was significantly greater (P < or = 0.05) in males for CON and INT. Results suggest that sweat rate differences do not affect sweat lactate concentrations between genders.
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