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Interaction of nitrous oxide and propofol to reduce hypertensive response to stimulation
1Department of Dental Anesthesiology, Tokyo Dental College, Chiba, Japan. ichinohe@tdc.ac.jp
Purpose:
To evaluate the interaction between nitrous oxide and propofol for the suppression of hypertension following electrical stimulation of the mental nerve in the rabbit.
Methods:
Male Japan White rabbits were tracheostomized, cannulated and mechanically ventilated under isoflurane anesthesia. Square wave pulses (5 V, 0.5 msec, 50 Hz for 5 sec) were delivered to the left mental nerve. Animals received nitrous oxide 20, 40, 60 and 80% (Group 1); propofol 200, 400, 600 and 800 microg x kg(-1) min(-1) (Group 2); or combinations of nitrous oxide and propofol at 10 + 100, 20 + 200, 30 + 300 and 40 % + 400 microg x kg(-1) x min(-1) (Group 3). Systolic blood pressure was recorded from immediately before to maximal increase following nerve stimulation. Probit analysis was used to obtain ED(50) values for 50% suppression of blood pressure elevation. Isobolographic analysis was used to evaluate the interaction between nitrous oxide and propofol.
Results:
ED(50) values are 52.9% for nitrous oxide (Group 1), 464.1 microg x kg(-1) min(-1) for propofol (Group 2), 21.7 % + 217.1 microg x kg(-1) min(-1) for nitrous oxide and propofol combination (Group 3) and 24. 7 % + 247.1 microg x kg(-1) x min(-1) for the theoretically additive combination of nitrous oxide and propofol, respectively.
Conclusion:
The interaction between nitrous oxide and propofol for the suppression of blood pressure elevation following electrical stimulation of the mental nerve is additive.