Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The interaction between Ran and NTF2 is required for cell cycle progression.

B B Quimby1, C A Wilson, A H Corbett

  • 1Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA.

Molecular Biology of the Cell
|August 10, 2000
PubMed
Summary

Nuclear transport factor 2 (NTF2) is essential for importing the small GTPase Ran into the nucleus. Insufficient nuclear Ran disrupts the cell cycle, causing a G2 arrest via the spindle assembly checkpoint.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immature teratoma of the ovary in children and adolescents: A multi-institutional study of characteristics and management.

Gynecologic oncology·2026
Same author

Immature teratoma of the ovary with microscopic yolk sac tumor in children and adolescents: A multi-institutional study.

Gynecologic oncology·2026
Same author

Stakeholder Support for Regulatory Harmonization and Expanded Nuclear Power: Outcomes of HPS/NCRP Open Forums.

Health physics·2026
Same author

Challenges and opportunities of the COVID-19 pandemic for perinatal mental health care: a mixed-methods study of mental health care staff.

Archives of women's mental health·2021
Same author

Increased risk of postpartum depression following severe obstetric lacerations in women with low resilience.

BJOG : an international journal of obstetrics and gynaecology·2020
Same author

Data for semi-permanent cationic coating for protein separations.

Data in brief·2020

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • The small GTPase Ran regulates nuclear transport and mitotic spindle assembly.
  • Ran exists in GTP-bound (nuclear) and GDP-bound (cytoplasmic) states.
  • Nuclear transport factor 2 (NTF2) facilitates the import of GDP-bound Ran (RanGDP) into the nucleus.

Purpose of the Study:

  • To investigate the in vivo role of NTF2 in nuclear Ran import.
  • To determine the consequences of impaired Ran nuclear import on cell cycle progression.

Main Methods:

  • Utilized a temperature-sensitive (ts) mutant of Saccharomyces cerevisiae NTF2 lacking Ran-binding activity.
  • Observed cell cycle progression and nuclear Ran import at non-permissive temperatures.

Related Experiment Videos

Main Results:

  • The NTF2 ts mutant failed to import Ran into the nucleus at the non-permissive temperature.
  • Cells with inefficient nuclear Ran import arrested in the G2 phase of the cell cycle.
  • This G2 arrest was dependent on the MAD2 checkpoint, indicating activation of the spindle assembly checkpoint.

Conclusions:

  • NTF2 is indispensable for the in vivo transport of Ran into the nucleus.
  • Depletion of nuclear Ran triggers a cell cycle arrest mediated by the spindle assembly checkpoint.