Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cellular actin is affected by interaction with Candida albicans.

I Tsarfaty1, H Sandovsky-Losica, L Mittelman

  • 1Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.

FEMS Microbiology Letters
|August 10, 2000
PubMed
Summary

Candida albicans interaction with human cells triggers significant actin cytoskeleton rearrangements. This study reveals how the opportunistic pathogen affects host cell structure and motility via secreted factors.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predicted no effect concentrations of antifungals for wastewater management and agricultural use.

Frontiers in toxicology·2026
Same author

Prediction of small-for-gestational age and fetal growth restriction at routine ultrasound examination at 35-37 weeks' gestation.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2025
Same author

Environmental and bioclimatic factors influencing yeasts and molds distribution along European shores.

The Science of the total environment·2022
Same author

Mycosands: Fungal diversity and abundance in beach sand and recreational waters - Relevance to human health.

The Science of the total environment·2021
Same author

Evidence for cutaneous dysbiosis in dystrophic epidermolysis bullosa.

Clinical and experimental dermatology·2021
Same author

Dissociation and other clinical phenomena in youth with psychogenic non-epileptic seizures (PNES) compared to youth with epilepsy.

Seizure·2019

Area of Science:

  • Microbiology
  • Cell Biology
  • Pathogenesis

Background:

  • * Candida albicans is an opportunistic pathogen causing infections through host tissue attachment.
  • * Understanding host-pathogen interactions at the cellular level is crucial for infection control.
  • * The role of mammalian cell cytoskeleton, particularly actin, during Candida interaction is not fully understood.

Purpose of the Study:

  • * To investigate the cellular events in mammalian cells following interaction with Candida albicans.
  • * To specifically examine the role and changes in the actin cytoskeleton.
  • * To characterize the effects of Candida-secreted factors on host cell actin dynamics.

Main Methods:

  • * Exposure of HEp2 human cell line to Candida albicans and its culture filtrate (arcsf).

Related Experiment Videos

  • * Confocal laser scanning microscopy (CLSM) with fluorescently labeled C. albicans and actin staining.
  • * Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) for ultrastructural analysis.

    • Main Results:

    • * C. albicans adhered to and was internalized by HEp2 cells, with surrounding actin.
    • * Actin rearrangement observed from filamentous networks to aggregates within HEp2 cells.
    • * Candida-secreted factor (arcsf) induced actin rearrangement, reduced membrane ruffling, and decreased motility in a dose- and time-dependent manner.

    Conclusions:

    • * Candida albicans interaction with HEp2 cells involves host cell signaling pathways.
    • * The cellular actin cytoskeleton is significantly affected during Candida infection.
    • * Candida-secreted factors play a key role in modulating host cell actin dynamics and cellular functions.