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Observation of apigenin anionic clusters in the gas phase
Rapid Communications in Mass Spectrometry : RCM
|August 10, 2000
Summary
Researchers developed a liquid chromatography/tandem mass spectrometry method to analyze apigenin, a flavonoid. They observed unique cluster ions, suggesting new methods for specific analysis of medicinal compounds.
Area of Science:
- * Analytical Chemistry
- * Phytochemistry
Background:
- * Flavonoids possess significant medicinal properties and are widely distributed.
- * Accurate quantification of flavonoids like apigenin in various matrices is crucial.
Purpose of the Study:
- * To develop and optimize a liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for apigenin analysis.
- * To investigate the behavior of apigenin under LC/MS/MS conditions, including the formation of unusual ions.
Main Methods:
- * Development of a liquid chromatography/tandem mass spectrometry (LC/MS/MS) method.
- * Analysis of the flavonoid apigenin in various matrices.
- * Characterization of observed oligomers and cluster ions using tandem mass spectrometry.
Main Results:
- * Formation of anionic oligomers of apigenin was observed during analysis.
- * Nitrate- and chloride-bound clusters of apigenin were identified.
- * Tandem mass spectrometry confirmed the cleavage of apigenin molecules from these cluster ions.
Conclusions:
- * The observed cluster ions indicate potential for novel analytical approaches.
- * Post-column derivatization techniques could be explored to enhance the specificity of apigenin analysis.
- * This study contributes to the precise determination of flavonoid levels in complex samples.
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