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[Application of gas chromatography in the identification of Enterobacter cloacae, Enterobacter aerogenes, and
E Robles Valderrama1, P Ramírez García, M E González Arreaga
1Unidad de Investigación Interdisciplinaria en Ciencias d e la Salud y la Educación, UNAM Campus Iztacala, México. erobles@servidor.unam.mx
Abstract:
Enterobacter cloacae, Enterobacter aerogenes and Enterobacter agglomerans were identified using gas chromatography as a substitution of the traditional techniques. Their acid methyl esters profiles were determined using a gas chromatograph Hewlett Packard 5890A and a RSL-150 heliflex capillary column. A total of 120 samples were analyzed from reference strains (ATCC 13047, 13048, 27155) and environmental isolations, eleven fatty acids were included in the profiles from which cis-9, 10-methyleneoctadecanoic acid (peak 24), cis-9-hexadecenoic acid (peak 14), octadecanoic acid (peak 23) and dodecanoic acid (peak 3), were the most important for the differentiation of the three species analyzed.