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[In vitro predatory activity of 8 fungal isolates against the nematode Panagrellus redivivus]
J Flores Crespo1, D Herrera Rodríguez, V Vázquez Prats
1Centro Nacional de Investigaciones Disciplinarias en Parasitología Veterinaria, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, SAGAR, Morelos, México.
Abstract:
The aim of this study was to evaluate the predacious capacity in vitro of eight isolates of nematophagous fungi: four of Arthrobotrys sp., one of Arthrobotrys oligospora, one of Duddingtonia flagrans, one of Dactylaria sp. and one Monacrosporium eudermatum. Nine groups of Petri dishes with 13 repetitions each were set up. The fungi were seeded in fluor-corn-agar media, following this each Petri dish was added with 150 larvae of the free living nematode Panagrellus redivivus. Five days after larval addition these were collected by Baermannization and were quantified. A significant difference (p < 0.05) between all treated group was observed respect with the control. Isolates FTHO-8 D. flagrans, R6 M. eudermatum, DAC Dactylaria sp. as well as FTHO-4 and FTHO-6 Arthrobotrys sp., showed an excellent predatory activity (> 90%) and they could be considered as potential bio-control agents in future field trials.
Insights
Eight nematophagous fungi isolates were tested for their ability to prey on Panagrellus redivivus nematodes. Several isolates, including Duddingtonia flagrans and Arthrobotrys sp., demonstrated over 90% predatory activity, indicating potential for biological control.
Area of Science:
- Agricultural Science
- Mycology
- Nematology
Background:
- Nematodes, particularly free-living species like Panagrellus redivivus, can impact agricultural ecosystems.
- Nematophagous fungi offer a promising avenue for biological control of plant-parasitic nematodes.
Purpose of the Study:
- To evaluate the in vitro predatory capacity of eight different nematophagous fungi isolates.
- To identify specific isolates with high predatory efficiency for potential use in biological control strategies.
Main Methods:
- Eight isolates of nematophagous fungi (Arthrobotrys sp., A. oligospora, Duddingtonia flagrans, Dactylaria sp., M. eudermatum) were cultured.
- Petri dishes containing fungi were inoculated with 150 larvae of Panagrellus redivivus.
- Larvae were quantified after five days using Baermannization to assess fungal predation.
Main Results:
- All tested fungi isolates showed significant predatory activity compared to the control group (p < 0.05).
- Isolates FTHO-8 (D. flagrans), R6 (M. eudermatum), DAC (Dactylaria sp.), FTHO-4, and FTHO-6 (Arthrobotrys sp.) exhibited excellent predatory activity exceeding 90%.
Conclusions:
- Specific isolates of nematophagous fungi possess high in vitro predatory capabilities against P. redivivus.
- These highly effective isolates are strong candidates for further investigation as biological control agents in field trials.