The expression of GABA(A) receptor subunits in the substantia nigra is developmentally regulated and region-specific

The substantia nigra pars reticulata (SNR) controls the spread of seizures. GABA(A)ergic drug (agonist or antagonist) infusions into the SNR have age-specific and site-specific effects on flurothyl-induced seizures. Developmental and cell-specific regulation of GABA(A) receptor subunit expression may be responsible for these specific effects. To test this hypothesis, in situ hybridization was used to examine regional expression of alpha1 and gamma2L GABA(A) receptor subunit mRNAs in the SNR during development. Distinct temporal and spatial patterns of expression were observed. In rats at postnatal days (PN) 21-60, fewer neurons were labeled with probes directed to alpha1 and gamma2L subunits in SNRanterior compared with SNRposterior. In addition, neurons in SNRanterior contained higher amounts of hybridization grains than in SNRposterior. In PN 15 rats, the labeling of neurons was relatively diffuse throughout the anterior and posterior SNR regions with moderate amounts of hybridization grains for both subunits. The finding of age-related differential distribution of alpha1 and gamma2L subunit mRNAs in the SNR suggests that GABA(A) receptor heterogeneity may play a role in the age-specific and site-specific effects of GABA(A)ergic agents on seizures in the SNR.