Development and optimisation of a flow injection assay for fluticasone propionate using an asymmetrical design and...

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis

Background:

• Fluticasone propionate is a widely used corticosteroid.
• Accurate quantification is crucial for pharmaceutical quality control.
• Existing analytical methods may require optimization for sensitivity and specificity.

Purpose of the Study:

• To develop and optimize a novel flow injection analysis (FIA) method for fluticasone propionate determination.
• To compare experimental designs for method optimization.
• To investigate optimal conditions for both UV and fluorescence detection.

Main Methods:

• Flow injection analysis (FIA) coupled with o-phthalaldehyde reaction chemistry.
• Optimization using experimental design: screening (fractional factorial vs. asymmetrical) and variable-size simplex algorithm.
• Dual detection capabilities: UV and fluorescence.

Main Results:

• The asymmetrical experimental design identified significant factors and confirmed local optima more effectively than the two-level fractional factorial design.
• Optimal parameters differed significantly between UV and fluorescence detection.
• Critical parameters for UV detection included temperature and on-line pH adjustment (NaOH, boric acid).
• Critical parameters for fluorescence detection included temperature and methanol fraction.

Conclusions:

• A robust FIA method for fluticasone propionate determination was successfully developed and optimized.
• Experimental design strategies significantly impact optimization outcomes.
• The distinct optimal conditions for UV and fluorescence detection provide flexibility for analytical applications.