Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lentiviral vectors: regulated gene expression.

T Kafri1, H van Praag, F H Gage

  • 1Laboratory of Genetics, The Salk Institute, La Jolla, California 92037, USA.

Molecular Therapy : the Journal of the American Society of Gene Therapy
|August 10, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Low-frequency genetic variants in GAK enhance Golgi function and protect against Parkinson's disease.

medRxiv : the preprint server for health sciences·2025
Same author

The effect of supraphysiological dose of nandrolone decanoate administration on the inflammatory, neurotrophin and behavioral response in adult and old male mice.

Hormones and behavior·2024
Same author

An in vitro model of lissencephaly: expanding the role of DCX during neurogenesis.

Molecular psychiatry·2017
Same author

Neurons derived from patients with bipolar disorder divide into intrinsically different sub-populations of neurons, predicting the patients' responsiveness to lithium.

Molecular psychiatry·2017
Same author

JNK1 controls adult hippocampal neurogenesis and imposes cell-autonomous control of anxiety behaviour from the neurogenic niche.

Molecular psychiatry·2017
Same author

JNK1 controls adult hippocampal neurogenesis and imposes cell-autonomous control of anxiety behaviour from the neurogenic niche.

Molecular psychiatry·2016
Same journal

Neonatal systemic gene therapy restores cardiorespiratory function in a rat model of Pompe disease.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same journal

Endothelial injury with capillary leak: A final common pathway in acute AAV toxicity?

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same journal

Long-term functional synaptic integration of genome-edited retinal organoids in a primate model of macular degeneration.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same journal

Nox1/4 inhibitor Setanaxib treatment ameliorates cardiac function in mouse models of Duchenne Muscular Dystrophy.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same journal

HSP90α-USP7-DNMT1 Axis Drives Hepatocellular Carcinoma Recurrence After Microwave Ablation by Disrupting ACSS3-Mediated Propionate Metabolism.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same journal

Cell-penetrating asymmetric siRNA targeting MyD88 suppresses inflammasome-driven ocular degeneration and angiogenesis.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
See all related articles

This study introduces a novel inducible lentiviral vector system for regulated gene delivery. This system allows for precise control of transgene expression in various cell types, including neurons, both in vitro and in vivo.

Area of Science:

  • Molecular Biology
  • Gene Therapy
  • Virology

Background:

  • Lentiviral vectors are effective for gene delivery into diverse cell types, including non-dividing cells.
  • Controlling gene expression is crucial for safe and effective gene therapy applications.

Purpose of the Study:

  • To develop and characterize a tetracycline (Tet)-inducible lentiviral vector system for regulated gene expression.
  • To demonstrate the in vitro and in vivo controllability of transgene expression using this novel vector.

Main Methods:

  • Generation of a lentiviral vector incorporating the Tet-regulated system.
  • In vitro transduction of human 293 cells and assessment of GFP expression.
  • In vivo transduction of rat brains and evaluation of doxycycline-mediated gene regulation.

Related Experiment Videos

Main Results:

  • The inducible lentiviral vector demonstrated tightly regulated GFP expression in human cells, with over 500-fold induction upon doxycycline withdrawal.
  • Gene expression could be switched on and off effectively, with maximal suppression achieved within 24 hours.
  • Doxycycline-regulated GFP expression was successfully observed in terminally differentiated neurons in the rat brain in vivo.

Conclusions:

  • An inducible lentiviral vector system enables precise control of transgene expression in vivo.
  • Regulated gene expression is a critical component for advancing gene therapy strategies.
  • This system holds significant potential for future therapeutic applications requiring controlled gene delivery.