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Development of surgical confocal scanning microscope for intra-operative imaging of brain tumors using near infrared
K Sakatani1, M Kashiwasake-Jibu, H Terada
1Department of Neurosurgery, China-Japan Friendship Hospital, Beijing, China. sakatani@public.east.cn.net
Abstract:
We developed a confocal laser scanning microscope for intra-operative imaging of brain tumors using near infrared fluorescence. The quality of near infrared images of Indocyanine Green (ICG) was compared with the surgical confocal scanning (SCS) microscope and a conventional charge-coupled device (CCD) camera; we compared images of a tube filled with ICG, which was located in the mouse brain. Compared to the CCD camera, the SCS microscope could obtain a more precise image of ICG fluorescence through the brain tissue. In addition, the SCS microscope could image ICG fluorescence clearly in a relatively light room because of elimination of stray light, while the CCD camera required high darkness to obtain ICG images. The present SCS microscope can give useful intra-operative imaging of brain tumors, particularly detection of residual tumor tissues that extend into normal brain tissues.
Insights
A new surgical confocal scanning (SCS) microscope offers precise intra-operative near-infrared fluorescence imaging of brain tumors using Indocyanine Green (ICG). This advanced microscope excels in detecting residual tumor tissues, even in lighter environments.
Area of Science:
- Neurosurgery
- Medical Imaging
- Optical Microscopy
Background:
- Accurate intra-operative imaging is crucial for complete brain tumor resection.
- Near-infrared fluorescence imaging with Indocyanine Green (ICG) aids in visualizing tumor margins.
- Limitations exist in current imaging techniques regarding precision and environmental light sensitivity.
Purpose of the Study:
- To develop and evaluate a confocal laser scanning microscope for intra-operative brain tumor imaging.
- To compare the performance of the novel surgical confocal scanning (SCS) microscope with a conventional charge-coupled device (CCD) camera for ICG fluorescence imaging.
Main Methods:
- Development of a confocal laser scanning microscope for near-infrared fluorescence imaging.
- Comparative analysis of ICG fluorescence imaging in mouse brain tissue using the SCS microscope and a CCD camera.
- Assessment of image quality, precision, and performance under varying light conditions.
Main Results:
- The SCS microscope provided more precise ICG fluorescence images through brain tissue compared to the CCD camera.
- The SCS microscope successfully imaged ICG fluorescence in a relatively light room by eliminating stray light.
- The CCD camera required complete darkness to obtain comparable ICG images.
Conclusions:
- The developed SCS microscope is a valuable tool for intra-operative brain tumor imaging.
- It enables clear visualization of ICG fluorescence, aiding in the detection of residual tumor tissues.
- The SCS microscope's performance in varied lighting conditions enhances its utility in surgical settings.