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Related Experiment Videos

CD31 immunoreactivity in small round cell tumors.

S A Nicholson1, M B McDermott, B R DeYoung

  • 1Lauren V. Ackerman Laboratory of Surgical Pathology, Washington University School of Medicine, St. Louis, Missouri, USA.

Applied Immunohistochemistry & Molecular Morphology : AIMM
|August 11, 2000
PubMed
Summary

CD31 is a marker for endothelial cells, but its role in small round cell tumors was unclear. This study found CD31 reactivity in some Ewing

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Area of Science:

  • Oncology
  • Immunohistochemistry
  • Pathology

Background:

  • CD31 is a known marker for endothelial differentiation in human neoplasms.
  • The diagnostic utility of CD31 in small round cell tumors remained unevaluated.

Purpose of the Study:

  • To investigate the role and diagnostic significance of CD31 expression in various small round cell tumors.
  • To determine if CD31 can aid in differentiating between lymphoid and non-lymphoid small round cell neoplasms.

Main Methods:

  • Immunohistochemical staining for CD31 was performed on 276 formalin-fixed, paraffin-embedded small round cell tumors.
  • Tumor types included Ewing's sarcoma/primitive neuroectodermal tumors, rhabdomyosarcomas, neuroblastomas, and various lymphomas.
  • A modified avidin-biotin-peroxidase complex technique with microwave epitope retrieval was utilized.

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Main Results:

  • CD31 reactivity was observed in a small subset (4/85) of Ewing's sarcoma/primitive neuroectodermal tumors.
  • No other non-lymphoid small round cell tumors showed CD31 positivity.
  • The majority of well-differentiated, intermediately differentiated, and lymphoblastic lymphomas were CD31 positive, while small cleaved lymphomas showed variable reactivity and small noncleaved lesions were negative.

Conclusions:

  • CD31 expression in Ewing's sarcoma/primitive neuroectodermal tumors is uncommon but possible.
  • CD31 positivity in a small cell neoplasm does not exclusively indicate hematopoietic origin.
  • These findings refine the understanding of CD31's utility in evaluating human neoplasms.