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A new insertion sequence, ISCb1, from Clostridium beijernickii NCIMB 8052.

H Liyanage¹, P Holcroft, V J Evans

¹Institute of Biological Sciences, University of Wales, Aberystwyth, Ceredigion, UK.

Journal of Molecular Microbiology and Biotechnology

|August 11, 2000

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A novel insertion sequence, ISCb1, found in Clostridium beijerinckii, shows characteristics of bacterial transposases. Despite its presence, ISCb1 is not linked to strain degeneration in solvent-forming clostridia.

Area of Science:

Microbiology
Genetics
Molecular Biology

Background:

Clostridium beijerinckii is known for solvent production.
Strain degeneration, a loss of solvent-forming potential, is a concern in industrial applications.
Insertion sequences (IS) are mobile genetic elements that can influence bacterial genomes.

Purpose of the Study:

To characterize a novel insertion sequence, ISCb1, in Clostridium beijerinckii.
To investigate the potential role of ISCb1 in strain degeneration.

Main Methods:

DNA sequencing and analysis of ISCb1 element.
Comparison of ISCb1 codon usage with other C. beijerinckii genes.
Analysis of ISCb1 profiles in wild-type and mutant strains.

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Main Results:

Nine copies of the 1764 bp ISCb1 element were identified in C. beijerinckii NCIMB 8052.
ISCb1 contains a 1365 bp open reading frame (ORF) encoding a putative transposase with conserved domains.
Codon usage suggests potential horizontal gene transfer of ISCb1.
No evidence links ISCb1 to strain degeneration; spontaneous mutants showed similar ISCb1 profiles to wild-type.

Conclusions:

ISCb1 is a novel IS4 family insertion sequence in Clostridium beijerinckii.
ISCb1 is not implicated in the degeneration of solvent-forming ability in these clostridia.
The element appears to be specific to closely related solvent-forming clostridia.