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IFN-gamma and LPS-mediated IL-10-dependent suppression of retinal microglial activation
C Broderick1, L Duncan, N Taylor
1Department of Ophthalmology, University of Aberdeen, United Kingdom.
Purpose:
Human retinal microglia (MG) express constitutively major histocompatability complex (MHC) class II molecules and have thus been highlighted as potential immunocompetent antigen-presenting cells (APCs). This study was undertaken to characterize microglial expression of coaccessory molecules and the functional changes in antigen expression, cytokine production, migration, and phagocytosis after proinflammatory stimulation.
Methods:
Fresh donor retinal MG were obtained and isolated using a percoll density gradient technique. Phenotypic characteristics used for isolating rodent microglia were applied and modified. Coaccessory molecule expression and intracellular cytokine production were assessed using three-color flow cytometric analysis in both freshly isolated and interferon (IFN)gamma-lipopolysaccharide (LPS)-stimulated MG. Using five-millimeter retinal explants in culture, microglial migratory behavior, changes in cell surface antigen expression and phagocytic activity were documented.
Results:
MG could be clearly defined by the flow cytometric phenotype CD45low CD11b+ MHC class II+ CD86low CD40low. Freshly isolated MG showed mannose receptor-mediated uptake of dextran-FITC. MG migrated from explants, were adherent, and upregulated MHC class II expression. After IFNgamma-LPS stimulation of single-cell suspension of MG isolates, MHC class II expression was reduced, with an increase occurring in MG intracellular interleukin (IL)-10 and IL-10 production. Microglial migration from explants was reduced after IFNgamma-LPS stimulation.
Conclusions:
These results highlight both phenotypic and behavioral characteristics that support an antigen-processing and -presenting capability of freshly isolated MG. However, proinflammatory stimulation with IFNgamma-LPS induces an IL-10-mediated downregulation of cell surface antigen expression and loss of migratory and phagocytic activity. Therefore, although equipped to act as APCs, MG are able to rapidly modulate their own function and behavior and as a result may have the potential to limit inflammation.
Insights
Human retinal microglia (MG) are antigen-presenting cells (APCs) that can limit inflammation. Proinflammatory stimulation reduces their antigen expression, migration, and phagocytosis, highlighting their dynamic regulatory role.
Area of Science:
- Immunology
- Neuroscience
- Ophthalmology
Background:
- Human retinal microglia (MG) constitutively express major histocompatibility complex (MHC) class II molecules.
- This expression suggests potential immunocompetent antigen-presenting cell (APC) capabilities in the retina.
Purpose of the Study:
- To characterize microglial coaccessory molecule expression.
- To investigate functional changes in antigen expression, cytokine production, migration, and phagocytosis following proinflammatory stimulation.
Main Methods:
- Retinal MG isolated using Percoll density gradient.
- Flow cytometry used to assess coaccessory molecule and cytokine expression.
- Retinal explants used to study migration, antigen expression, and phagocytosis.
Main Results:
- Freshly isolated MG exhibit mannose receptor-mediated uptake and migrate from explants.
- Proinflammatory stimulation (IFNγ-LPS) reduced MHC class II expression but increased IL-10 production.
- Stimulation decreased microglial migration and phagocytic activity.
Conclusions:
- Freshly isolated MG possess phenotypic and behavioral characteristics of APCs.
- Proinflammatory stimulation induces IL-10-mediated downregulation of function.
- MG can modulate their activity, potentially limiting inflammation.