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Related Experiment Videos

Rapid quantitative bioassay of osteoinduction.

H D Adkisson1, J Strauss-Schoenberger, M Gillis

  • 1Department of Medicine, Barnes-Jewish Hospital/Washington University, St. Louis, Missouri 63110, USA.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
|August 11, 2000
PubMed
Summary

A new bioassay using Saos cells quantifies the osteoinductive potential of demineralized bone matrix. This method reliably predicts new bone formation, aiding in screening bone allograft donors.

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Orthopedic Research

Background:

  • Demineralized bone matrix (DBM) is crucial for bone regeneration.
  • Assessing the osteoinductive capacity of DBM is vital for clinical applications.
  • Current methods for evaluating osteoinduction can be time-consuming or lack reproducibility.

Purpose of the Study:

  • To develop and validate a rapid, reproducible bioassay for quantifying the osteoinductive capacity of human DBM.
  • To correlate in vitro cell proliferation with in vivo bone formation.
  • To establish a screening tool for bone allograft donor selection.

Main Methods:

  • A bioassay utilizing Saos human osteosarcoma cell proliferation in response to DBM was developed.
  • Osteoinduction was assessed in vivo by implanting DBM into athymic rats and evaluating new bone formation after 35 days.

Related Experiment Videos

  • Correlation analysis was performed between the Saos cell proliferation index and the quantitative measure of new bone formation.

    • Main Results:

    • Saos cell proliferation demonstrated a significant correlation (r=0.850, p < 0.0005) with in vivo osteoinduction across 25 DBM samples.
    • The bioassay showed acceptable interassay (23 +/- 2%) and intra-assay (11 +/- 1%) variability.
    • Distinct low, intermediate, and high osteoinductive index groups were established with no overlap in osteoinduction between low and high groups.

    Conclusions:

    • The Saos cell proliferation bioassay is a reliable and quantitative method for assessing the osteoinductive potential of human DBM.
    • This assay can be used for routine screening of bone allograft donors.
    • The assay provides a valuable tool for quality control in the production of bone graft materials.