Monoclonal antibodies to Rh D--development and uses

Area of Science:

• Immunology
• Hematology
• Biotechnology

Background:

• Monoclonal anti-D antibody production has been challenging in rodent systems.
• Human monoclonal anti-D has been successfully generated using Epstein-Barr virus (EBV) transformed B cells and myeloma fusions.
• Recent advancements utilize molecular biology techniques for monoclonal anti-D production.

Purpose of the Study:

• To review the range of available monoclonal anti-D antibodies.
• To discuss the selection criteria for monoclonal anti-D in blood grouping reagents for donors and recipients.
• To consider the development of monoclonal anti-D for prophylactic use.

Main Methods:

• Production of human monoclonal anti-D using EBV-transformed peripheral B cells fused with myeloma cell lines.
• Application of molecular biology techniques for generating monoclonal anti-D.
• Review and analysis of existing monoclonal anti-D products and their applications.

Main Results:

• The study considers the spectrum of monoclonal anti-D antibodies currently produced.
• Guidelines are presented for selecting monoclonal anti-D for typing, emphasizing all D-positive donors should be typed as positive.
• A specific recommendation is made to type DVI patients as D-negative.

Conclusions:

• Monoclonal anti-D antibodies are vital for accurate blood grouping.
• Careful selection is necessary for optimal donor and recipient typing, including special considerations for DVI individuals.
• Further development of monoclonal anti-D for prophylactic applications is warranted.