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Related Experiment Videos

Flow cytometric analysis of platelets.

A D Michelson1

  • 1Center for Platelet Function Studies, University of Massachusetts Medical School, Worcester, USA. michelson@platelets.org

Vox Sanguinis
|August 12, 2000
PubMed
Summary
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International journal of laboratory hematology·2014

New flow cytometry methods allow in vivo study of platelet function. These findings suggest P-selectin measurement in stored platelets is not a reliable predictor of in vivo platelet survival or function.

Area of Science:

  • Hematology
  • Transfusion Medicine
  • Immunology

Background:

  • Studying platelet function in vivo is challenging despite advances in in vitro methods.
  • Existing techniques are insufficient for comprehensive in vivo platelet analysis.

Purpose of the Study:

  • To develop novel methods for studying platelet function in vivo.
  • To track infused platelets and measure their functional capacity within a living organism.

Main Methods:

  • Development of a three-color whole blood flow cytometry assay.
  • Application of the assay for in vivo tracking and functional assessment of platelets.

Main Results:

  • Circulating platelets expressing P-selectin (indicating degranulation) were observed to rapidly shed P-selectin into plasma.

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  • Despite P-selectin loss, these platelets remained functional and continued to circulate.

    • Conclusions:

    • P-selectin levels on stored platelet concentrates do not accurately predict in vivo platelet survival or function.
    • The developed flow cytometry methods offer a powerful tool for investigating in vivo platelet behavior in transfusion medicine.