Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hydrodynamic flow profiling in microchannel structures by single molecule fluorescence correlation spectroscopy

Gosch1, Blom, Holm

  • 1Department of Medical Biophysics, Karolinska Institute, Stockholm, Sweden.

Analytical Chemistry
|August 12, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of food deprivation and handling stress on head kidney 17alpha-hydroxyprogesterone 21-hydroxylase activity, plasma cortisol and the activities of liver detoxification enzymes in rainbow trout.

Aquatic toxicology (Amsterdam, Netherlands)·2001
Same author

Effect of anisotropy on the instability of crack propagation

Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics·2000
Same author

Strong attraction between charged spheres due to metastable ionized states

Physical review letters·2000
Same author

Theoretical investigation of high pressure phases of carbon dioxide

Physical review letters·2000
Same author

Functional immobilisation of the nicotinic acetylcholine receptor in tethered lipid membranes

Biophysical chemistry·2000
Same author

Protecting Groups for Thiols Suitable for Suzuki Conditions.

Organic letters·2000
Same journal

Machine Learning-Assisted Label-Free SERS Decoding of Mitochondrial Molecular Dynamics in Ovarian Granulosa Cells during Aging.

Analytical chemistry·2026
Same journal

Revealing the Regulatory Interplay of NHE1 mRNA and Na<sup>+</sup> in Cancer Cells Using a DNA Nanosensor.

Analytical chemistry·2026
Same journal

Towards Cellular Resolution of Tryptic Peptides in Tissue Sections by MALDI MS Imaging: A Focus on Enzyme Application and Reproducibility.

Analytical chemistry·2026
Same journal

Bioinspired Bilayer Hydrogel Colorimetric Sensor Array for Low-Temperature Food Freshness Analysis.

Analytical chemistry·2026
Same journal

Quartz Crystal Microbalance-Based Point-of-Care Testing Systems: Principles, Device Design, and Applications.

Analytical chemistry·2026
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
See all related articles

This study presents high spatial resolution hydrodynamic flow profiling in silicon microchannels using single molecule fluorescence correlation spectroscopy (FCS). The technique successfully mapped parabolic Poiseuille flow profiles within microchannels, enabling future non-destructive analysis of microstructures.

Area of Science:

  • Microfluidics
  • Biophysics
  • Analytical Chemistry

Background:

  • Microfluidic devices are crucial for various applications, requiring precise characterization of internal fluid dynamics.
  • Understanding flow profiles in microchannels is essential for optimizing device performance and experimental outcomes.
  • Traditional methods for flow profiling can be invasive or lack the necessary spatial resolution.

Purpose of the Study:

  • To demonstrate high spatial resolution hydrodynamic flow profiling in silicon wafer-based microchannels.
  • To utilize single molecule fluorescence correlation spectroscopy (FCS) for detailed flow analysis.
  • To validate the measured flow profiles against established fluid dynamics principles.

Main Methods:

  • Employing confocal fluorescence microscopy with single tetramethylrhodamine (TMR-4-dUTP) biomolecules as tracers.

Related Experiment Videos

  • Defining a small (approx. 1 fL) observation volume using an argon laser focus.
  • Scanning the microchannel (50 x 50 µm²) in 1-µm steps vertically and horizontally to map the flow.
  • Inducing continuous hydrodynamic flow by elevating an analyte reservoir.

    • Main Results:

    • Successfully achieved high spatial resolution hydrodynamic flow profiling within microchannels.
    • Detected single biomolecules traversing the defined laser focus volume.
    • The measured flow profiles exhibited a parabolic shape in both vertical and horizontal dimensions.
    • Confirmed the presence of Poiseuille laminar flow within the silicon microchannels.

    Conclusions:

    • Single molecule fluorescence correlation spectroscopy (FCS) is a viable technique for high-resolution hydrodynamic flow profiling in microchannels.
    • The demonstrated method provides non-destructive characterization of microfluidic flow.
    • This technique offers potential for future investigations and quality control of microfluidic devices and other microstructures.