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Rapid and direct sequencing of double-stranded DNA using exonuclease III and MALDI-TOF MS
U Puapaiboon1, J Jai-nhuknan, J A Cowan
1Evans Laboratory of Chemistry, The Ohio State University, Columbus 43210, USA.
Analytical Chemistry
|August 12, 2000
Abstract:
Application of MALDI-TOF MS to direct sequencing of dsDNA substrates is demonstrated using a strategy that employs exonuclease III digestion of a target sequence. Experimental conditions for exonuclease III have been optimized for this application, including addition of essential divalent metal ion cofactors. A short cation-exchange column was designed to provide efficient sample cleanup and overcome major problems arising from salt interference.