Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

A rapid fluorescence based multiplex polymerase chain reaction--single-strand conformation polymorphism method for

P Berggren¹, G Steineck, K Hemminki

¹Department of Biosciences at NOVUM, Karolinska Institute, Huddinge, Sweden. petra.berggren@cnt.ki.se

Electrophoresis

|August 12, 2000

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Correction: Risk of hernia formation after radical prostatectomy: a comparison between open and robot-assisted laparoscopic radical prostatectomy within the prospectively controlled LAPPRO trial.

Hernia : the journal of hernias and abdominal wall surgery·2022

Same author

Risk of hernia formation after radical prostatectomy: a comparison between open and robot-assisted laparoscopic radical prostatectomy within the prospectively controlled LAPPRO trial.

Hernia : the journal of hernias and abdominal wall surgery·2020

Same author

Mindfetalness to increase women's awareness of fetal movements and pregnancy outcomes: a cluster-randomised controlled trial including 39 865 women.

BJOG : an international journal of obstetrics and gynaecology·2020

Same author

Identifying female pelvic cancer survivors with low levels of physical activity after radiotherapy: women with fecal and urinary leakage need additional support.

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2019

Same author

Physical activity before radical prostatectomy reduces sick leave after surgery - results from a prospective, non-randomized controlled clinical trial (LAPPRO).

BMC urology·2016

Same author

Pursuing Quality in the Application of Bladder Cancer Quality of Life Research.

Bladder cancer (Amsterdam, Netherlands)·2016

This study introduces a rapid single-strand conformation polymorphism (SSCP) method for detecting mutations in the p53 gene. The technique efficiently screens for genetic alterations in cancer samples, offering high sensitivity and specificity.

Area of Science:

Molecular Biology
Genetics
Cancer Research

Background:

The p53 tumor suppressor gene is crucial in preventing cancer.
Detecting mutations in p53 is vital for cancer diagnosis and treatment.
Existing mutation screening methods can be time-consuming and complex.

Purpose of the Study:

To develop and evaluate a fast, sensitive method for screening p53 gene mutations.
To improve the efficiency of mutation detection in cancer-related genes.

Main Methods:

Utilized multiplex polymerase chain reaction (PCR) to amplify exons 5-8 of the p53 gene.
Employed fluorescent single-strand conformation polymorphism (SSCP) for mutation screening.
Validated the technique using known cell lines and human urinary bladder cancer samples, followed by sequencing.

Related Experiment Videos

Main Results:

The developed SSCP technique detected 21/23 mutations in cell lines and 10/10 mutations in patient samples.
Achieved high sensitivity (91-100%) and specificity (88-97%) across cell lines and patient samples.
Demonstrated a sensitivity of 87% (cell lines) and 90% (patient samples) using a single SSCP gel.

Conclusions:

The modified SSCP technique is an efficient and highly sensitive tool for detecting p53 gene mutations.
This method offers a rapid and reliable approach for genetic screening in cancer research.
The technique shows potential for widespread application in clinical diagnostics and molecular epidemiology.