Role of fucosyltransferases in the association between apomucin and Lewis antigen expression in normal and malignant gastric epithelium
- A López-Ferrer 1, C de Bolós , C Barranco , M Garrido , J Isern , I Carlstedt , C A Reis , J Torrado , F X Real
- 1Unitat de Biologia Cel.lular i Molecular, Institut Municipal d'Investigació Mèdica, Universitat Pompeu Fabra, Barcelona, Spain.
- 0Unitat de Biologia Cel.lular i Molecular, Institut Municipal d'Investigació Mèdica, Universitat Pompeu Fabra, Barcelona, Spain.
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View abstract on PubMed
Summary
This summary is machine-generated.Gastric cancer development involves changes in mucin glycosylation, driven by fucosyltransferases, not apomucin sequences. Intestinal metaplasia shows early signs of these altered cellular phenotypes.
Area Of Science
- Gastroenterology
- Molecular Biology
- Cancer Research
Background
- Normal gastric epithelium exhibits distinct MUC5AC and MUC6 mucin expression patterns associated with specific Lewis antigens.
- The stomach serves as a model to study how glycosyltransferases influence apomucin glycosylation specificity.
Purpose Of The Study
- To elucidate the molecular basis of mucin-antigen associations in the stomach.
- To track changes in gastric and intestinal apomucin expression and Lewis antigen association during gastric carcinogenesis.
Main Methods
- Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect fucosyltransferase (FUT1-3) and mucin (MUC5AC, MUC6) transcripts.
- Immunohistochemistry and in situ hybridization analyzed apomucin (MUC2, MUC4, MUC5AC, MUC6) and Lewis antigen expression.
Main Results
- FUT1 and FUT2 expression showed co-regulation with MUC6 and MUC5AC, respectively, in normal gastric tissue, a pattern lost in tumors.
- Gastric tumors, particularly those with an intestinal phenotype, displayed upregulation of MUC2 and MUC4, with coexpression of gastric and intestinal mucins.
- These mucin expression changes were identified as early events in gastric carcinogenesis, present even in intestinal metaplasia.
Conclusions
- Cellular fucosyltransferase expression, not apomucin sequence, dictates glycosylation patterns in normal gastric epithelium.
- Gastric cancer development and intestinal metaplasia are characterized by the emergence of cellular phenotypes not found in normal gastric tissue.
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