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Estimation of stem cell fractions in peripheral blood stem cell harvest by using an SE-9000 hematology analyzer
K Saigo1, T Sugimoto, S Takeuchi
1Blood Transfusion Division, Kobe University Hospital, Kobe, Japan. saigo@med.kobe-u.ac.jp
Abstract:
We inquired whether stem cell fractions in peripheral blood stem cell harvest could be precisely detected by using a hematopoietic progenitor cell (HPC) counting system applied to an automated hematology analyzer, SE-9000. Although there was an apparent increase in the HPCs 20 h after storage in nondiluted conditions, samples diluted with RPMI-1640 containing 1.0 mg/ml EDTA-2K showed a relatively stable number of HPCs. There was a significant relationship between HPCs and CD34 cells (n = 75, r = 0.769). This method may represent the least expensive and most time-effective way for stem cell estimation in harvest products.