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Published on: September 26, 2014
Isolation and properties of human alpha-fetoprotein from HepG2 cell cultures
H F Deutsch1, N Taniguchi, M A Evenson
1Department of Biomolecular Chemistry, Laboratory Medicine and Medicine, University of Wisconsin, Madison, WI 53706, USA. hdeutsch@facstaff.wisc.edu
Abstract:
A relatively rapid 3-step fractionation method has been developed for the isolation of human alpha-fetoprotein from culture fluids of HepG2 cells applicable to large volumes. The protein exists as a complex with lipids or lipoproteins but an ethanol precipitation step is effective in separating it. Yields of 50-60% can be obtained from culture fluid containing 30-40 microg/ml. A minor fraction that appears to be a proteolytic product of the AFP is present in the final product.
