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Related Experiment Videos

Transmissible substrate-utilizing ability in enterobacteria.

H W Smith, Z Parsell

    Journal of General Microbiology
    |March 1, 1975
    PubMed
    Summary
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    Bacterial conjugation can transfer traits like sucrose utilization (Sac+) and raffinose utilization (Raf) between Escherichia coli and other bacteria. However, these acquired traits may reduce bacterial survival in the chicken gut.

    Area of Science:

    • Microbiology
    • Genetics
    • Bacterial Physiology

    Background:

    • Bacterial conjugation is a key mechanism for horizontal gene transfer.
    • Understanding the transmissibility of metabolic traits is crucial for microbial ecology and evolution.
    • Escherichia coli and Salmonella species are important model organisms and pathogens.

    Purpose of the Study:

    • To investigate the transmissibility of sucrose (Sac+) and raffinose utilization (Raf) abilities in Escherichia coli.
    • To determine if these traits are co-transferred with other factors like K88 antigen and enterotoxin production.
    • To assess the impact of acquired metabolic traits on bacterial fitness in a host environment.

    Main Methods:

    • Bacterial conjugation experiments were performed to transfer Sac+ and Raf+ abilities.

    Related Experiment Videos

  • Characterization of transmissible elements and their association with K88 antigen and enterotoxin production.
  • Fitness assays in the alimentary tract of chickens to compare parent and transconjugant strains.

    • Main Results:

    • Sucrose utilization (Sac+) was transferred by conjugation in 3 of 152 E. coli strains; the transfer factor was thermosensitive in some cases.
    • Raffinose utilization (Raf) was transmissible in 27 of 163 E. coli strains, often co-transferred with K88 antigen and enterotoxin production determinants.
    • Lactose, salicin, and dulcitol utilization abilities were not transferable under the tested conditions.
    • Acquisition of Sac+ or Raf+ traits led to reduced survival in the chicken alimentary tract compared to parent strains.

    Conclusions:

    • Metabolic traits like sucrose and raffinose utilization are transferable via conjugation in E. coli and can be established in other species including Salmonella and Shigella.
    • Co-transfer of metabolic traits with virulence factors like K88 antigen and enterotoxin suggests potential for rapid adaptation in pathogenic bacteria.
    • Acquisition of these metabolic capabilities may impose a fitness cost on bacteria in specific environments, such as the chicken gut.