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Updated: Oct 13, 2026

In Situ Hybridization for the Precise Localization of Transcripts in Plants
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In Situ Hybridization for the Precise Localization of Transcripts in Plants

Published on: November 23, 2011

An improved nonfluorescent detection system for in situ hybridization in plants

H Weiss1, P Pasierbek, J Maluszynska

  • 1Department of Plant Anatomy and Cytology, Silesian University, Kotawice, Poland.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
|August 15, 2000
PubMed

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...