Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Analysis of brush-particle interactions using self-consistent-field theory.

B M Steels1, J Koska, C A Haynes

  • 1Department of Chemical and Bioresource Engineering and the Protein Engineering Network of Centers of Excellence, The University of British Columbia, Vancouver, Canada.

Journal of Chromatography. B, Biomedical Sciences and Applications
|August 15, 2000
PubMed
Summary

Polymer chains grafted to surfaces reduce protein adsorption and aid bioseparations. Modeling shows chain length and density influence solute interaction energy, predicting repulsion due to entropy loss or attraction via favorable contacts.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of saxagliptin on adipose tissue inflammation and vascular function in overweight and obese people: a placebo-controlled study.

Diabetic medicine : a journal of the British Diabetic Association·2018
Same author

<i>In vivo</i> study and thermodynamic investigation of two lanthanum complexes, La(dpp)<sub>3</sub> and La(XT), for the treatment of bone resorption disorders.

Chemical science·2018
Same author

Salsalate has no effect on insulin secretion but decreases insulin clearance: a randomized, placebo-controlled trial in subjects without diabetes.

Diabetes, obesity & metabolism·2015
Same author

Stabilities of intact hemoglobin molecules and hemoglobin peptides in dried blood samples.

Clinica chimica acta; international journal of clinical chemistry·2013
Same author

A randomised trial of salsalate for insulin resistance and cardiovascular risk factors in persons with abnormal glucose tolerance.

Diabetologia·2013
Same author

Performance of succinylacetone assays and their associated proficiency testing outcomes.

Clinical biochemistry·2012

Area of Science:

  • Surface Science
  • Polymer Chemistry
  • Biophysics

Background:

  • Non-specific protein adsorption on surfaces is a challenge in bioseparations.
  • End-grafted polymer layers are used to modify surface properties for applications like chromatography.

Purpose of the Study:

  • To understand how to tailor polymer-modified surfaces for bioseparation applications.
  • To investigate the influence of polymer chain characteristics and solute interactions on surface properties.

Main Methods:

  • Numerical self-consistent field (SCF) model to calculate polymer density distributions.
  • Statistical mechanical treatment of a lattice model to determine interaction energy.
  • Systematic variation of parameters: particle size, chain length, surface density, and Flory interaction parameters.

Related Experiment Videos

Main Results:

  • Polymer segments rapidly fill space behind a moving solute particle.
  • Repulsive interaction energy is observed due to conformational entropy loss in the absence of polymer-solute attraction.
  • A maximum repulsive force occurs above a critical chain length due to particle engulfment.
  • Highest surface density optimizes solute repulsion when polymer-solute attraction exists.

Conclusions:

  • Surface-grafted polymers can be tailored to control solute interactions for bioseparation.
  • Understanding polymer-solute energetics and chain conformation is key to designing effective surfaces.
  • Longer chains can increase solute concentration if favorable polymer-solute contacts outweigh entropic penalties.