New aqueous two-phase system based on cashew-nut tree gum and poly(ethylene glycol)
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Updated: Oct 13, 2026

Cell Co-culture Patterning Using Aqueous Two-phase Systems
Published on: March 26, 2013
L A Sarubbo1, L A Oliveira, A L Porto
1DESQ-FEQ, UNICAMP, Campinas-SP-Brasil, Brazil.
Abstract:
The characterisation of a new system based on cashew-nut tree gum, a branched acidic heteropolysaccharide found in Brazil, and poly(ethylene glycol) (PEG) was studied. Phase diagrams are provided for the PEG-cashew-nut tree gum system. The influence of PEG molecular mass, tie-line length and pH on bovine serum albumin (BSA) partition was investigated. Protein partition coefficient was little influenced by changing PEG molecular mass. Increasing the tie-line length decreased the partition. Increasing the pH also raised the BSA partition coefficient. It is shown that systems formed by PEG and cashew-nut tree gum may be considered as an interesting alternative for use in protein purification.
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