Related Experiment Video

Updated: Jul 31, 2026

Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
11:56

Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species

Published on: April 17, 2009

A protocol for PCR in situ hybridization of hyphomycetes

K Sterflinger1, W E Krumbein, A Schwiertz

  • 1Geomicrobiology, ICBM, Carl von Ossietzky Universität, Oldenburg, Germany. Sterflinger@biologie.uni-oldenburg.de

International Microbiology : the Official Journal of the Spanish Society for Microbiology
|December 8, 2000
PubMed

More Related Videos

In Situ Hybridization for the Precise Localization of Transcripts in Plants12:15

In Situ Hybridization for the Precise Localization of Transcripts in Plants

Published on: November 23, 2011

Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples09:38

Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples

Published on: November 23, 2016

Related Concept Videos

Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...