Histological examination of CAM treated with irritative pesticides

The potential irritancy of compounds may be detected by observing adverse changes which occur in the chorioallantoic membrane (CAM) of the egg after exposure to tested chemicals. In our experiment irritating pesticides (Fusilade S, Karathene LC) and a technical component of pesticide (Trend) are tested and their effects are examined on tissue structure of CAM. After 10-11 days of incubation of chicken embryos the chorioallantoic membrane becomes trilaminar. The outer layer is chorionic epithelium between the two rows of cuboidal cells, thin-walled vascular sinuses can be found. In the intermediate layer, the mesenchyme, well-developed blood vessels can be seen. The inner layer is formed by allantoic epithelium. After the treatment with Fusilade S the wall of the blood vessels was damaged, and blood diffused from the blood vessels. After the treatment with Karathane LC rupture of the wall of the blood vessels could be seen and blood appeared around the blood vessels. Blood vessels of the yolk sac were frequently damaged under CAM.