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In vitro colonisation of acrylic resin denture base materials by Streptococcus oralis and Actinomyces viscosus
A S Kagermeier-Callaway1, B Willershausen, T Frank
1Poliklinik für Zahnerhaltungskunde, Mainz, Germany.
Aim:
The aim of the study was to compare the attachment of two typical strains of oral bacteria to four denture base materials.
Design:
In vitro study.
Method:
Discs of acrylic resin denture base materials (Paladon 65, polished and unpolished; Palapress; Microbase, polished and unpolished, and Triad VLC) were placed into Petri dishes with Schaedler's medium, inoculated with Streptococcus oralis 34 or Actinomyces viscosus T14V.
Main Outcome Measures:
After 24 h or 48 h the numbers of adhering bacteria were measured.
Results:
The bacteria adhered to all discs in similar numbers: 3-9 x 10(6)/ml (viable cell count) and 9-22 x 10(8)/ml (total cell count) for T14V, and 2-6 x 10(6)/ml (viable cell count) and 1.5-3 x 10(8)/ml (total cell count) for 34.
Conclusions:
Polishing had little effect on adherence. Denture base materials are not resistant against adherence and possible surface damage by oral bacteria. Therefore, thorough oral hygiene is important for denture wearers.