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Related Experiment Videos

Low frequency of replication errors in primary nervous system tumours.

M J Sobrido1, C R Pereira, F Barros

  • 1Department of Neurology, Complexo Hospitalario Universitario de Santiago, Santiago de Compostela, Spain. msobrido@ucla.edu

Journal of Neurology, Neurosurgery, and Psychiatry
|August 17, 2000
PubMed
Summary

Microsatellite instability (MIN) is rare in adult primary CNS tumors, with a true replication error phenotype being uncommon. MIN was more frequent in tetranucleotide repeats and associated with spinal schwannomas.

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Area of Science:

  • Neuro-oncology
  • Molecular genetics
  • Cancer research

Background:

  • Microsatellite instability (MIN) is a genetic alteration observed in various cancers.
  • Its role in primary central nervous system (CNS) tumors is not well-defined.
  • Automated DNA technology offers precise analysis of genetic instability.

Purpose of the Study:

  • To investigate the incidence of MIN in common adult primary CNS tumors.
  • To correlate MIN with clinicopathological characteristics.
  • To assess the significance of the replication error (RER+) phenotype.

Main Methods:

  • Automated DNA analysis using fluorescent polymerase chain reaction (PCR) and fragment analysis.
  • Screening of 56 gliomas, 32 meningiomas, and 11 schwannomas for microsatellite size changes.

Related Experiment Videos

  • Defining MIN+ (instability in ≥1 locus) and RER+ (instability in ≥25% of loci) phenotypes.

    • Main Results:

    • Overall instability rate was 2.47%, higher in tetranucleotide than dinucleotide repeats.
    • MIN+ tumors: 17.9% gliomas, 6.3% meningiomas, 18.2% schwannomas.
    • RER+ phenotype was uncommon; MIN associated with shorter clinical course in meningiomas and spinal schwannomas.

    Conclusions:

    • Microsatellite instability occurs at a low rate in primary CNS tumors.
    • A widespread replication error phenotype is rare and unlikely to be a major pathogenic factor.
    • This study is the first to report MIN in schwannomas, noting an association with spinal tumors.