Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

T cell effector function and anergy avoidance are quantitatively linked to cell division.

A D Wells1, M C Walsh, D Sankaran

  • 1Department of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|August 18, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Use of acidified versus non-acidified liquid human milk fortifier in very low birth weight infants: A retrospective comparison of clinical outcomes.

Journal of neonatal-perinatal medicine·2019
Same author

Dietary supplementation with flaxseed meal and oat hulls modulates intestinal histomorphometric characteristics, digesta- and mucosa-associated microbiota in pigs.

Scientific reports·2018
Same author

Comparative effects of two multi-enzyme combinations and a Bacillus probiotic on growth performance, digestibility of energy and nutrients, disappearance of non-starch polysaccharides, and gut microflora in broiler chickens.

Poultry science·2017
Same author

Effects of a multi-strain <i>Bacillus</i> spp. direct-fed microbial and a protease enzyme on growth performance, nutrient digestibility, blood characteristics, fecal microbiota, and noxious gas emissions of grower pigs fed corn-soybean-meal-based diets-A meta-analysis.

Journal of animal science·2017
Same author

Barriers to enrollment in a randomized controlled trial of hydrocortisone for cardiovascular insufficiency in term and late preterm newborn infants.

Journal of perinatology : official journal of the California Perinatal Association·2017
Same author

Flaxseed meal and oat hulls supplementation modulates growth performance, blood lipids, intestinal fermentation, bile acids, and neutral sterols in growing pigs fed corn-soybean meal-based diets.

Journal of animal science·2017
Same journal

Optineurin restrains IL-17-associated neuroinflammation in trigeminal ganglia to preserve sensory function after ocular HSV-1 infection.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Crystal structure and immune single-cell atlas provide insights into the functional divergence of type I IFNs in fish.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Complement C3 deficiency increases the effector and cytotoxic functions of NK cells and suppresses tumor growth.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Increased Nur77 is disconnected from TCR affinity in insulin-specific Tregs.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

FTR85 negatively regulates type I IFN antiviral signaling pathway by promoting K48-linked polyubiquitination of IRF3.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

An MR1-specific nanobody capable of blocking MR1T cell activation.

Journal of immunology (Baltimore, Md. : 1950)·2026
See all related articles

Cell division history dictates T cell function. T cells that divide more proliferate more, while non-dividing cells become unresponsive, highlighting cell division

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • T cells show significant variability in proliferation after activation.
  • A substantial portion of activated T cells do not expand clonally.

Purpose of the Study:

  • To investigate how prior cell division impacts the function of primary T cells.
  • To understand the single-cell behavior of T cells based on their division history.

Main Methods:

  • Primary CD4+ T cells were stimulated and sorted by division number.
  • Sorted cells were restimulated via T cell receptor (TCR) and CD28 ligation.
  • Analyzed IL-2 production, proliferation, and signaling pathways.

Main Results:

  • T cells with more division rounds showed increased IL-2 production and proliferation upon restimulation.

Related Experiment Videos

  • Non-dividing T cells entered a hyporesponsive state, resistant to proliferative signals.
  • Anergy in non-dividing cells was linked to defects in MAPK/ERK and p27kip1 regulation, but not calcium flux or STAT5 activation.

    • Conclusions:

    • Cell division is crucial for preventing T cell anergy.
    • Cell cycle progression is integral to forming the effector/memory T cell pool.
    • Division history shapes T cell responsiveness and fate.