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Related Experiment Videos

Characterization of Porphyromonas gingivalis insertion sequence-like element ISPg5.

J V Califano1, T Kitten, J P Lewis

  • 1Department of Periodontics, Virginia Commonwealth University, Richmond, Virginia 23298, USA. califano@vcu.edu

Infection and Immunity
|August 19, 2000
PubMed
Summary

Porphyromonas gingivalis exhibits strain diversity, potentially driven by insertion sequence (IS)-like elements. A novel IS element, ISPg5, was identified and may be useful for P. gingivalis strain fingerprinting.

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Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Porphyromonas gingivalis is a key pathogen in periodontitis.
  • Significant variability exists among P. gingivalis strains, suggesting genetic mechanisms for diversity.
  • Intragenomic changes, such as insertion sequence (IS) elements, are potential drivers of this variability.

Purpose of the Study:

  • To investigate the occurrence and characteristics of IS-like elements in Porphyromonas gingivalis strain W83.
  • To identify novel repetitive DNA sequences within the P. gingivalis genome.
  • To explore the role of IS elements in P. gingivalis strain diversity.

Main Methods:

  • Adaptation of a DNA sequence capture method to isolate repetitive elements.
  • Analysis of the P. gingivalis W83 genome sequence, focusing on CTAG tetranucleotide clusters.

Related Experiment Videos

  • Identification and characterization of a novel IS-like element, designated ISPg5.
  • Southern analysis of various P. gingivalis strains using a recombinant plasmid containing ISPg5.

    • Main Results:

    • Isolation and cloning of IS-like sequences, including known IS elements and a novel element.
    • Discovery of 11 copies of the novel IS-like element (ISPg5) within the P. gingivalis W83 genome.
    • ISPg5, a 1,512-bp element, shares features with the IS3 family of IS elements.
    • Southern analysis revealed apparent diversity among P. gingivalis strains, suggesting differential distribution of ISPg5.

    Conclusions:

    • Novel insertion sequence elements, like ISPg5, are present in Porphyromonas gingivalis.
    • These IS elements likely contribute to the genetic diversity observed among P. gingivalis strains.
    • ISPg5 and other IS elements show potential for use in P. gingivalis strain fingerprinting and epidemiological studies.