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[Epidermal growth factor-responsive precursors with similar properties isolated from embryonic rat septum and
L Castillo1, E Grygar, B Hutter-Paier
1Centro Internacional de Restauración Neurológica, La Habana, Cuba. lazara@neubas.sld.cu
Introduction:
The potential correspondence of precursor cells isolated from different brain regions is partially unknown. Since models and culture conditions used in several studies vary, comparison of precursor characteristics has been limited.
Objective:
In this paper epidermal growth factor (EGF)-responsive precursors from the striatum and septum were isolated and their growth pattern in vitro were determined. We also evaluated the influence of fibroblast growth factor (FGF) and nerve growth factor (NGF) on the proliferation of these cells.
Material And Methods:
Dissociated cells from rat septum and striatum were cultivated in suspension with 20 ng/ml of EGF. Total cells quantification, immunocytochemical staining and neuron counts were used to evaluate cell proliferation and cellular phenotypes produced by EGF-generated cells.
Results:
Considering both culture evolution and cellular growth we demonstrated a similar growth pattern of septal and striatal EGF-responsive precursors. Furthermore, the proliferation of both cells populations was supported by FGF. On the contrary, NGF neither had a proliferative effect nor affected cell survival. Upon differentiation, a small proportion of precursor cells differentiated into neuronal phenotype.