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Related Experiment Videos

Macrophages in bovine milk.

D L Jensen, R J Eberhart

    American Journal of Veterinary Research
    |May 1, 1975
    PubMed
    Summary

    Bovine mammary gland cells, typically called epithelial cells, were found to exhibit macrophage-like properties. These vacuolated mononuclear cells phagocytosed bacteria, suggesting they are indeed macrophages.

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    Area of Science:

    • Veterinary immunology
    • Cell biology
    • Bovine mammary gland physiology

    Background:

    • Mammary gland secretions contain various cell types.
    • Mononuclear cells in bovine milk are often misidentified.
    • Understanding these cells' function is crucial for mammary health.

    Purpose of the Study:

    • To characterize the functional properties of vacuolated mononuclear cells in normal bovine milk.
    • To determine if these cells possess macrophage-like characteristics.
    • To clarify the identity of these commonly observed milk cells.

    Main Methods:

    • In vitro assays assessing neutral red uptake, adherence, and spreading on glass.
    • In vitro phagocytosis assays using live Streptococcus agalactiae and Staphylococcus aureus.
    • In vivo assessment of neutral red uptake and phagocytosis of formalin-killed Staphylococcus aureus.

    Main Results:

    • Vacuolated mononuclear cells concentrated neutral red in vitro and in vivo.
    • These cells adhered rapidly and spread on glass surfaces.
    • Phagocytosis of both live and killed bacteria (Streptococcus agalactiae and Staphylococcus aureus) was observed.

    Conclusions:

    • The studied vacuolated mononuclear cells exhibit key functional properties of macrophages.
    • These findings suggest a reclassification of these cells from epithelial cells to macrophages.
    • This identification has implications for understanding bovine mammary gland immunity and health.

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