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Review of calcium methodologies.

B Zak, E Epstein, E S Baginski

    Annals of Clinical and Laboratory Science
    |May 1, 1975
    PubMed
    Summary

    This review classifies serum calcium analytical systems, recommending a direct reaction method using cresolphthalein complexone to minimize errors for accurate calcium measurement.

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    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry

    Background:

    • Serum calcium measurement is crucial for diagnosing various health conditions.
    • Numerous analytical methodologies for serum calcium have evolved over the past century.

    Purpose of the Study:

    • To review and classify historical and current serum calcium methodologies.
    • To recommend an optimized, direct reaction method for serum calcium analysis.

    Main Methods:

    • Classification of analytical systems from gravimetry to isotope dilution-mass spectrometry.
    • Discussion of screening and referee procedures.
    • Evaluation of atomic absorption spectrophotometry and molecular absorption spectrophotometry sensitivities.
    • Development of a direct reaction method using cresolphthalein complexone with dimethylsulfoxide to minimize blanks.

    Main Results:

    • Comprehensive overview of evolving serum calcium analytical techniques.
    • Identification of comparative sensitivities between different spectrophotometric methods.
    • Demonstration of a method to minimize high blanks in serum calcium determination.
    • Inclusion of reaction characteristics, potential errors, and normal ranges.

    Conclusions:

    • The reviewed methodologies provide a historical perspective on serum calcium analysis.
    • The recommended direct reaction method offers an efficient and accurate approach for serum calcium determination.
    • Understanding analytical system evolution aids in selecting appropriate diagnostic tools.

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