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Mediator-nucleosome interaction
Y Lorch1, J Beve, C M Gustafsson
1Department of Structural Biology, Stanford School of Medicine, Stanford, California 94305, USA.
Molecular Cell
|August 19, 2000
Abstract:
Mediator, a multiprotein complex involved in the regulation of RNA polymerase II transcription, binds to nucleosomes and acetylates histones. Three lines of evidence identify the Nut1 subunit of Mediator as responsible for the histone acetyltransferase (HAT) activity. An "in-gel" HAT assay reveals a single band of the appropriate size. Sequence alignment shows significant similarity of Nut1 to the GCN5-related N-acetyltransferase superfamily. Finally, recombinant Nut1 exhibits HAT activity in an in-gel assay.