Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure of poly (dT).poly (dA).poly (dT).

R Chandrasekaran1, A Giacometti, S Arnott

  • 1Whistler Center for Carbohydrate Research, Purdue University, West Lafayette, IN 47907, USA. chandra@purdue.edu

Journal of Biomolecular Structure & Dynamics
|August 19, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Acceptability of innovative male contraceptive methods among major of age heterosexual adult men in France in 2021].

Progres en urologie : journal de l'Association francaise d'urologie et de la Societe francaise d'urologie·2023
Same author

Is there a POST-COVID dermatological syndrome? The integrated dermato-infectious disease experience of a single centre.

Journal of the European Academy of Dermatology and Venereology : JEADV·2021
Same author

Tocilizumab in COVID-19 interstitial pneumonia.

Journal of internal medicine·2021
Same author

Discovery and synthesis of 2-amino-1-methyl-1H-imidazol-4(5H)-ones as GPCR ligands; an approach to develop breast cancer drugs via GPCR associated PAR1 and PI3Kinase inhibition mechanism.

Bioorganic chemistry·2019
Same author

Twelve-month prevalence and treatment gap for common mental disorders: Findings from a large-scale epidemiological survey in India.

Indian journal of psychiatry·2017
Same author

A Reduction Grade of Lipodystrophy and Limited Side Effects after HAART Regimen with Raltegravir, Lamivudine, Darunavir and Ritonavir in an HIV-1 Infected Patient after Six Years of Antiretroviral Therapy.

The West Indian medical journal·2015
Same journal

Anti-mycobacterial activity of phytocompounds from <i>Ricinus communis</i> L. - an integrated <i>in-vitro</i> and <i>in-silico</i> approach.

Journal of biomolecular structure & dynamics·2026
Same journal

Binding studies of the X-ray characterized [SnMe<sub>2</sub>Cl<sub>2</sub>(Me<sub>2</sub>phen)] complex with human serum albumin: experimental and molecular docking approaches.

Journal of biomolecular structure & dynamics·2026
Same journal

Computational design and experimental validation of peptide inhibitors to disrupt urease enzyme maturation in pathogenic bacteria <i>Proteus mirabilis</i>.

Journal of biomolecular structure & dynamics·2026
Same journal

Wavelet-domain multiway spectral separation of free drug, DNA, and drug-DNA complex profiles for quantitative binding analysis based on fractional occupancy (<i>θ</i>).

Journal of biomolecular structure & dynamics·2026
Same journal

Gene expression and microsecond scale conformational dynamics suggest potential regulatory mechanisms for the expanded subtilase family of <i>T. rubrum</i>.

Journal of biomolecular structure & dynamics·2026
Same journal

Deciphering the Role of Sugar Osmolytes in Free and Nano forms to Mitigate Protein Aggregation: Insights from Biophysical and Microscopic Studies.

Journal of biomolecular structure & dynamics·2026
See all related articles

Researchers determined the molecular structure of poly(dT).poly(dA).poly(dT) DNA. This triple helix features a 12-fold right-handed helix stabilized by four hydrogen bonds per base triplet.

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biophysics

Background:

  • Poly(dT).poly(dA).poly(dT) is a synthetic DNA construct.
  • Understanding DNA structures is crucial for molecular biology.
  • Triple-helical DNA structures offer unique biological properties.

Purpose of the Study:

  • To determine the precise molecular structure of poly(dT).poly(dA).poly(dT).
  • To refine the structural model using X-ray diffraction data.
  • To elucidate the hydrogen bonding and conformational details of the triple helix.

Main Methods:

  • X-ray diffraction analysis of oriented DNA fibers.
  • Continuous intensity data collection on layer lines.
  • Structure refinement using crystallographic methods.

Related Experiment Videos

Main Results:

  • A 12-fold right-handed triple helix structure was identified.
  • The helix has a pitch of 38.4 Å.
  • Each base triplet is stabilized by four Crick-Watson-Hoogsteen hydrogen bonds.
  • Deoxyribose rings exhibit C2'-endo conformations in all strands.
  • A grooveless cylindrical shape was observed.

Conclusions:

  • The determined structure provides a detailed model for this synthetic DNA triple helix.
  • The hydrogen bonding network and sugar pucker are key features of the structure.
  • The observed cylindrical shape correlates with the lack of lateral fiber organization.