Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Current applications of single-cell PCR.

S Hahn1, X Y Zhong, C Troeger

  • 1Department of Obstetrics and Gynaecology, University of Basel, Switzerland. shahn@uhbs.ch

Cellular and Molecular Life Sciences : CMLS
|August 19, 2000
PubMed
Summary

Polymerase chain reaction (PCR) enables genetic analysis of single cells, revolutionizing molecular biology. This technique is crucial for prenatal diagnostics and basic research, with future advances promising wider applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HnRNPA2B1 tunes antimycobacterial immune responses in macrophages through alternative splicing of <i>Irgm1</i>.

Infection and immunity·2026
Same author

Energy Spectrum of Ultrahigh-Energy Cosmic Rays across Declinations -90° to +44.8° as Measured at the Pierre Auger Observatory.

Physical review letters·2026
Same author

HnRNPA2B1 tunes antimycobacterial immune responses in macrophages through alternative splicing of <i>Irgm1</i>.

bioRxiv : the preprint server for biology·2025
Same author

Search for the Anomalous Events Detected by ANITA Using the Pierre Auger Observatory.

Physical review letters·2025
Same author

Inference of the Mass Composition of Cosmic Rays with Energies from 10^{18.5} to 10^{20}  eV Using the Pierre Auger Observatory and Deep Learning.

Physical review letters·2025
Same author

Exploring long-term cancer survivors' care experiences and unmet needs: protocol for a qualitative study.

BMC cancer·2024

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • The polymerase chain reaction (PCR) has transformed molecular biology by enabling the amplification of minute genetic material.
  • Single-cell analysis was previously limited but is now feasible, offering new diagnostic and research avenues.

Purpose of the Study:

  • To highlight the revolutionary impact of single-cell polymerase chain reaction (PCR) in molecular biology.
  • To discuss the applications of single-cell PCR in prenatal diagnostics and basic scientific research.
  • To explore the future potential of single-cell genetic analysis with recent technological advancements.

Main Methods:

  • Utilizes polymerase chain reaction (PCR) for amplifying genetic material from single cells.
  • Incorporates techniques like whole genome amplification (WGA) and complementary DNA (cDNA) arrays.

Related Experiment Videos

  • Enables examination of both genomic DNA and messenger RNA (mRNA) expression patterns.

    • Main Results:

    • Single-cell PCR facilitates preimplantation genetic analysis.
    • Fetal cells from maternal blood can be used for assessing Mendelian disorders.
    • Single-cell PCR aids in immunological, neurological, and developmental research by analyzing gene expression.

    Conclusions:

    • Single-cell genetic analysis is becoming standard practice due to technological progress.
    • Optimized WGA, single-cell cDNA arrays, and comparative genomic hybridization will expand capabilities.
    • These advancements open new frontiers for both genetic diagnosis and scientific discovery.