Related Experiment Videos
Chloramphenicol in aqueous humor after topical application
Archives of Ophthalmology (Chicago, Ill. : 1960)
|March 1, 1975
Summary
Topical chloramphenicol eye drops reach measurable levels in aqueous humor for up to five hours after administration. This study confirms intact chloramphenicol presence, not metabolites, in the eye before cataract surgery.
Area of Science:
- Ophthalmology
- Pharmacokinetics
- Drug Metabolism
Background:
- Chloramphenicol is an antibiotic used to treat eye infections.
- Understanding its penetration into ocular tissues is crucial for effective treatment and safety.
Purpose of the Study:
- To quantify chloramphenicol levels in human aqueous humor after topical ophthalmic application.
- To determine the duration of chloramphenicol presence in the anterior chamber.
- To identify whether intact chloramphenicol or its metabolites are present.
Main Methods:
- Chloramphenicol 0.5% ophthalmic solution was administered topically to patients prior to cataract surgery.
- Aqueous humor samples were collected during surgery.
- Thin-layer chromatography was used to analyze and quantify chloramphenicol content.
Main Results:
- Aqueous humor chloramphenicol concentrations ranged from 3.5 to 6.7 ng/mL one to two hours post-administration.
- Chloramphenicol was detectable in aqueous humor for up to five hours.
- Analysis confirmed the presence of intact chloramphenicol, with no metabolites detected.
Conclusions:
- Topical chloramphenicol ophthalmic solution achieves significant concentrations in the anterior chamber.
- The drug persists for several hours, indicating potential for sustained therapeutic effect.
- The absence of metabolites suggests minimal ocular metabolism of chloramphenicol.