Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A role for carnosine in cellular maintenance.

R Holliday1, G A McFarland

  • 1CSIRO Division of Molecular Science, North Ryde, Sydney, NSW 1670, Australia. RandL.Holliday@bigpond.com

Biochemistry. Biokhimiia
|August 22, 2000
PubMed
Summary

L-carnosine extends the lifespan of human fibroblasts in vitro, delaying aging and rejuvenating cells. However, it is cytotoxic to neoplastic cells without pyruvate.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tooth wear time-bomb.

British dental journal·2026
Same author

Targeted periodontitis management in ACS patients.

British dental journal·2025
Same author

Misleading and worrying.

British dental journal·2023
Same author

Vaping misrepresentations.

British dental journal·2022
Same author

Waterline Disinfectants Reduce Dental Bioaerosols: A Multitracer Validation.

Journal of dental research·2022
Same author

Electronic cigarettes: an update on products, regulation, public health approaches and oral health.

Community dental health·2022

Area of Science:

  • Cell biology
  • Biochemistry
  • Aging research

Background:

  • L-carnosine is a naturally occurring dipeptide with potential antioxidant and anti-glycation properties.
  • Cellular senescence is a complex process associated with aging and various diseases.
  • Understanding factors that modulate fibroblast lifespan and senescence is crucial for regenerative medicine and aging studies.

Purpose of the Study:

  • To investigate the effects of L-carnosine on the in vitro lifespan and senescence of human fibroblasts.
  • To determine the impact of L-carnosine on aged or senescent cells.
  • To assess the cytotoxicity of L-carnosine on neoplastic and transformed cells.

Main Methods:

  • Culturing human fibroblasts in media with and without L-carnosine at physiological concentrations.
  • Monitoring cell proliferation, morphology, and senescence markers.
  • Assessing the effects of L-carnosine withdrawal and addition on cell state.
  • Evaluating L-carnosine's cytotoxicity on neoplastic and transformed cells in the presence and absence of pyruvate.

Main Results:

  • Physiological concentrations of L-carnosine significantly prolonged the in vitro lifespan of human fibroblasts.
  • L-carnosine reduced key features of cellular senescence in cultured fibroblasts.
  • Late-passage (aged) fibroblasts were rejuvenated by transfer to L-carnosine-containing medium.
  • Removal of L-carnosine induced senescence in previously rejuvenated cells.
  • L-carnosine demonstrated cytotoxicity towards neoplastic and transformed human and rodent cells, but only in the absence of pyruvate.
  • The optical isomer, D-carnosine, did not exhibit these effects.

Conclusions:

  • L-carnosine possesses significant beneficial effects on human fibroblast cultures, extending lifespan and reversing senescence.
  • The anti-senescence properties of L-carnosine suggest potential therapeutic applications in aging and age-related diseases.
  • L-carnosine's differential effect on normal versus neoplastic cells, particularly its pyruvate-dependent cytotoxicity, warrants further investigation for cancer therapy.

Related Experiment Videos