Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hydration of pectic polysaccharides.

P Ryden1, A J MacDougall, C W Tibbits

  • 1Food Biopolymer Section, Institute of Food Research, Norwich Laboratory, Norwich Research Park, Colney, Norwich NR4 7UA, UK. Peter.Ryden@bbsrc.ac.uk

Biopolymers
|August 22, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Temporal and long-term gut microbiota variation in allergic disease: A prospective study from infancy to school age.

Allergy·2018
Same author

Erratum to: A new concept and a comprehensive evaluation of SYSMEX UF-1000i flow cytometer to identify culture-negative urine specimens in patients with UTI.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2017
Same author

A new concept and a comprehensive evaluation of SYSMEX UF-1000i  flow cytometer to identify culture-negative urine specimens in patients with UTI.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2017
Same author

High-risk regions and outbreak modelling of tularemia in humans.

Epidemiology and infection·2016
Same author

Gut microbiome and innate immune response patterns in IgE-associated eczema.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2015
Same author

Movement and metabolism of oligogalacturonide elicitors in tomato shoots.

Planta·2013

Pectic polysaccharide swelling is driven by ion movement, decreasing with higher salt or acidity. Calcium ions significantly reduce swelling and promote network formation in these biopolymers.

Area of Science:

  • Biochemistry
  • Polymer Science
  • Materials Science

Background:

  • Pectic polysaccharides are key components of plant cell walls.
  • Their hydration and swelling properties influence material structure and function.
  • Understanding these properties is crucial for applications in food, pharmaceuticals, and biomaterials.

Purpose of the Study:

  • To investigate the hydration and swelling behavior of pectic polysaccharides.
  • To determine the influence of pH, ionic strength, and counterions on swelling.
  • To elucidate the underlying mechanisms driving swelling under osmotic stress.

Main Methods:

  • Experiments were conducted at varying pH and ionic strengths.
  • Osmotic stress was applied to pectic polysaccharide films.

Related Experiment Videos

  • The effects of monovalent (Na+) and divalent (Ca2+) counterions were compared.

    • Main Results:

    • At low salt concentrations (20 mM Na+), swelling is driven by polyelectrolyte effects and ion translational entropy.
    • Increased salt concentration and lower pH reduce swelling.
    • Highly charged pectins exhibit collapse and minimal swelling.
    • Replacing Na+ with Ca2+ drastically reduces swelling and induces network formation, even in weakly charged pectins.

    Conclusions:

    • The swelling of pectic polysaccharides is highly sensitive to environmental conditions.
    • Ionic strength, pH, and the type of counterion are critical factors governing hydration.
    • Divalent cations like Ca2+ play a significant role in structuring pectin networks, limiting swelling.