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Related Experiment Videos

Cyclic AMP activates Ras.

O M Tsygankova1, E Kupperman, W Wen

  • 1Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia 19104-6084, USA.

Oncogene
|August 22, 2000
PubMed
Summary
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Cyclic adenosine monophosphate (cAMP) activates Ras protooncogene in thyroid cells, independent of protein kinase A (PKA). This cAMP-mediated Ras activation is crucial for TSH-stimulated proliferation, suggesting additional cAMP targets beyond PKA.

Area of Science:

  • Cellular signaling
  • Molecular biology
  • Endocrinology

Background:

  • Cyclic adenosine monophosphate (cAMP) is a key second messenger regulating various cellular processes.
  • Thyrotropin (TSH) stimulates thyroid cell proliferation via a cAMP-dependent pathway.
  • The role of Ras protooncogene in TSH-induced proliferation is known, but its precise regulation by cAMP is unclear.

Purpose of the Study:

  • To investigate whether cAMP targets include regulators of the Ras protooncogene.
  • To determine the role of Ras activity in cAMP-mediated thyroid cell proliferation.
  • To elucidate the relationship between cAMP, protein kinase A (PKA), and Ras activation.

Main Methods:

  • Using rat thyroid cells and TSH stimulation.
  • Measuring Ras activity (GTP-bound Ras) via Western blotting.

Related Experiment Videos

  • Employing cAMP-elevating agents and PKA inhibitors.
  • Assessing DNA synthesis and cell proliferation.

    • Main Results:

    • TSH rapidly and significantly increased Ras activity in a cAMP-dependent manner.
    • Ras activation by TSH and cAMP occurred independently of PKA activity.
    • Inhibition of Ras impaired TSH- and cAMP-stimulated DNA synthesis.
    • Microinjection of PKA catalytic subunit did not stimulate proliferation, unlike cAMP-elevating agents.

    Conclusions:

    • cAMP activates targets beyond PKA in thyroid cells, including regulators of Ras.
    • cAMP-mediated Ras activation is a critical downstream event in TSH-stimulated thyroid cell proliferation.
    • These findings explain why PKA alone cannot fully mediate cAMP's proliferative effects.