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Updated: Aug 13, 2026
Preparation of Cell-lines for Conditional Knockdown of Gene Expression and Measurement of the Knockdown Effects on E4orf4-Induced Cell Death
Published on: October 21, 2012
[Resistance of Bcl-2 adenovirus vector to HepG(2) cell apoptosis induced by ethanol]
1Institute of Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing 400010, China.
Objective:
To evaluate the role of Bcl-2 family proteins in hepatocytic apoptosis caused by ethanol.
Methods:
We induced hepatocytic apoptosis in HepG(2) cells with ethanol and detected the apoptosis with TUNEL assay. Bcl-2, Bax, and Bak protein expression in the hepatocytes was determined by immunohistochemistry method. We also observed the expression of Bcl-2 protein in HepG(2) cells infected with Bcl-2 adenovirus vector and its protection against hepatocytic apoptosis caused by ethanol.
Results:
No obvious cytotoxicity was noted in 0.2% and 1% ethanol treatment group, while it developed in 3% ethanol treatment group and accompanied by a marked expression of Bax, Bak proteins.
Conclusion:
The overexpression of Bax, Bak proteins may play a role in HepG(2) cell apoptosis induced by ethanol and can be blocked effectively by Bcl-2 adenovirus vector.
