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Updated: Aug 10, 2026

Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules
Published on: August 25, 2009
Patterning and characterization of surfaces with organic and biological molecules by the scanning electrochemical
I Turyan1, T Matsue, D Mandler
1Department of Molecular Chemistry and Engineering, Faculty of Engineering, Tohoku University, Sendai, Japan.
Abstract:
A novel approach for micropatterning of surfaces with organic and biological microstructures using the scanning electrochemical microscope (SECM) is described. The approach is based on the introduction of the spatial resolution by local deposition of gold particles followed by monolayer formation and functionalization. Specifically, gold patterns were deposited locally on silicon wafers with the SECM as a result of the controlled anodic dissolution of a gold microelectrode. The gold patterns were further used as microsubstrates for assembling cystamine monolayers to which either fluoresceine isothiocyanate (FIT) or glucose oxidase (GOD) were covalently attached. Characterization of the organic monolayers, as well as the biological activity of the enzyme patterns, was carried out by fluorescence microscopy and the SECM, respectively.
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