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Related Experiment Videos

ATP-induced [Ca(2+)](i) changes and depolarization in GH3 cells.

H S Chung1, K S Park, S K Cha

  • 1Department of Physiology, Yonsei University, Wonju College of Medicine, Wonju 220-701, Korea.

British Journal of Pharmacology
|August 22, 2000
PubMed
Summary

Extracellular adenosine triphosphate (ATP) activates P2X7 receptors in GH3 cells, influencing calcium signaling and cell permeability. This suggests a role for purinergic signaling in neuroendocrine modulation.

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Area of Science:

  • Neuroendocrinology
  • Cellular Signaling
  • Molecular Pharmacology

Background:

  • Extracellular adenosine triphosphate (ATP) acts as a neurotransmitter.
  • ATP plays a role in calcium (Ca2+) signaling and hormone secretion in the endocrine system.
  • The specific ATP receptor subtype in GH3 cells remained unclear.

Purpose of the Study:

  • To elucidate the ATP receptor subtype involved in GH3 cell responses.
  • To investigate the effects of purinergic stimulation on calcium signaling and cell permeability in GH3 cells.
  • To explore the potential role of purinergic signaling in neuroendocrine modulation.

Main Methods:

  • Utilized GH3 cells, a rat anterior pituitary cell line.
  • Measured intracellular calcium ([Ca2+]i) responses and cell depolarization.

Related Experiment Videos

  • Employed reverse transcriptase (RT)-polymerase chain reaction (PCR) to detect P2X7 mRNA.
  • Assessed cell permeability using ethidium bromide influx and NMDG permeability.

    • Main Results:

    • BzATP was a more potent agonist than ATP for both [Ca2+]i responses and depolarization.
    • Oxidized ATP (oATP) nearly abolished ATP- and BzATP-induced responses, indicating P2X7 receptor involvement.
    • BzATP induced non-desensitizing cationic currents and increased cell permeability to larger molecules.
    • P2X7 mRNA was identified in GH3 cells.

    Conclusions:

    • GH3 cells possess an endogenous P2X7 receptor.
    • Purinergic stimulation via P2X7 receptors influences calcium signaling and cell permeability in GH3 cells.
    • Purinergic signaling may play a significant role in neuroendocrine modulation.