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Updated: Aug 17, 2026

Experimental Metastasis Assay
Published on: August 24, 2010
Serial analysis of gene expression identifies putative metastasis-associated transcripts in colon tumour cell lines
A Parle-McDermott1, P McWilliam, O Tighe
1Department of Biochemistry, Royal College of Surgeons in Ireland, 123 St Stephen's Green, Dublin 2, Ireland.
Abstract:
We have used serial analysis of gene expression (SAGE) to identify gene expression differences between a primary colon tumour cell line (SW480) and an isogenic lymph-node metastasis cell line (SW620). Differential expression was confirmed for the following genes: keratin K5, cystatin S, serum amyloid A, the human homologue of yeast ribosomal S28 and the p32 subunit of human pre-mRNA splicing factor SF2. Expression of confirmed differences were also analysed in other metastatic cell lines.
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