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Updated: Oct 13, 2026
Bacterial Inner-membrane Display for Screening a Library of Antibody Fragments
Published on: October 15, 2016
Engineering and characterization of a single-chain antibody fragment (scFV1924) against the human sarcoma-associated
H Chen1, P Wang, T K Das Gupta
1Department of Surgical Oncology, University of Illinois, Chicago College of Medicine, USA.
Background:
Monoclonal Antibody (MAb) 19-24 against the human sarcoma-associated antigen (SAA) p102 was previously characterized, and it demonstrated potential for sarcoma immunodiagnosis and immunotherapy. Due to the limitations of using intact murine MAbs in human clinical settings, we engineered a single-chain antibody fragment of MAb 19-24 (scFV1924).
Materials And Methods:
Monoclonal Antibody (MAb) 19-24 against the human sarcoma-associated antigen (SAA) p102 was previously characterized, and it demonstrated potential for sarcoma immunodiagnosis and immunotherapy. Due to the limitations of using intact murine MAbs in human clinical settings, we engineered a single-chain antibody fragment of MAb 19-24 (scFV1924).
Results:
The results from competition radioimmunoassay showed that binding of MAb 19-24 to SAA p102 on sarcoma cell membranes was inhibited by scFV1924.
Conclusions:
This indicates the potential of further application of the scFV1924 in human sarcoma immunotherapy.
